Alkaliphiles: Genetic Properties and Applications of Enzymes by Koki Horikoshi

By Koki Horikoshi

The pioneering paintings of Dr. Koki Horikoshi at the discovery, study and functions of alkaliphilic micro organism are defined the following of their entirety. The isolation, distribution and taxonomy of alkaliphilic microorganisms in addition to their cellphone constitution and body structure are mentioned for a easy realizing of those entities. The molecular biology and genome sequencing of a few alkaliphilic bacterial traces also are presented.

Part II of the amount makes a speciality of enzymes of alkaliphiles and their purposes. those comprise alkaline proteases, starch-degrading enzymes and diverse others. a few of these enzymes are presently in vast advertisement use as laundry detergent ingredients and in wastewater treatment.

Alkaliphiles are a comparatively fresh box of analysis that may doubtless supply avenues to a variety of extra discoveries and functions for a brand new new release of staff in bioscience and technology.

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Many microorganisms have been isolated from these alkaline environments. More transient, localized alkaline environments due to animal excreta and such may exist in soils that are not alkaline overall. 2). Some alkaliphilic bacteria can change external pH value to a pH suitable for growth and create their own world, as described in the following section. Since 1968, Horikoshi and his coworkers have systematically studied alkaliphilic microorganisms (Horikoshi 1971a). They have paved the way in establishing a new microbiology, alkaliphilic microbiology, to use these microorganisms as microbial and genetic resources.

Biosci. Biotechnol. , 63, 943(1999b)) In 2005 Nogi et al. reported their extensive phylogenetic studies on alkaliphilic Bacillus strains. Unrooted phylogenetic tree of akaliphilic Bacillus strains isolate in the author's laboratory since 1968 is shown in Fig. 8. 3 Taxonomy of Alkaliphiles 21 B. hahdiirans ATCC 27557^ (AB021187) B. aAiM 1139'(AB043858) B krulwichiae JCM 1169P (AB086897) B. wakoensis N-F (AB0438S1) B pseudofirmus DSM 8715^ (X76439) B pseudofirmus 27-1 (AB043845) B pseudofirmus 8-1 (AB043842) B pseudofirmus IM (AB043857) B pseudofirmus 124-1 (AB201799) B pseudofirmus A-40-2 (AB201795) B gihsonii DSM 8722' (X76446) B patagoniemis DSM 16117' (AY258614) B oshimemisKM 12663'(AB18890) B.

2). Some alkaliphilic bacteria can change external pH value to a pH suitable for growth and create their own world, as described in the following section. Since 1968, Horikoshi and his coworkers have systematically studied alkaliphilic microorganisms (Horikoshi 1971a). They have paved the way in establishing a new microbiology, alkaliphilic microbiology, to use these microorganisms as microbial and genetic resources. 5 in Horikoshi-I medium. 0 and all indicated optimum pH value for growth above 8.

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