An Introduction to Microbiology. Pharmaceutical Monographs by W. B. Hugo, J. B. Stenlake

By W. B. Hugo, J. B. Stenlake

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Examples of energy-yielding reactions will be considered in a later section. It is possible to classify groups of bacteria on a basis which takes only the nature of their nutritional requirements and mode of obtaining energy into account. Two such schemes are given below. NUTRITIONAL CLASSIFICATION MICRO-ORGANISMS OF In the first place micro-organisms may be divided into two groups, autotrophs and heterotrophs, according to the nature of the carbon-containing derivatives they are able to utilise.

30 THE BACTERIAL CELL Acid-fast organisms appear red, non-acid-fast organisms appear blue. The classical example of an acid-fast organism is the causal organism of tuberculosis, Mycobacterium tuberculosis. Other members of the genus Mycobacterium, bacterial spores and many fungal spores are acid-fast and a similar technique can be used to differentiate spores from vegetative forms in a bacterial culture that might contain both. Acid-fastness in the mycobacteria has been attributed to the presence in the cells of waxes which strongly combine with the stain when heated with it.

Lb. arabinosus Staph. aureus + — — — + + * + + + ? + N. meningitidis Pr. vulgaris 1 1 Sal. typhosa I 1 Pr. morganii Es eh. coli 1 Glycine Alanine Serine Cystine Phenylalanine Tyrosine Tryptophan Threonine Valine Leucine Isoleucine Glutamic acid Aspartic acid Histidine Lysine Arginine Methionine Proline Hydroxyproline Glutamine Asparagine 1 BACTERTA — — — — — * 7 — — + — * + —+ — + + — — * + —+ — + + — — + — — — — — — ? — + — — — + —+ — — + — — — — — — + +7 ? 7 7 + + ——+ + + + + + _ 7 + + + +') + 7 _ — 9 — — 7 — + + — 7 — + + + — ?

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