Bacterial Toxins: Tools in Cell Biology and Pharmacology by Klaus Aktories

By Klaus Aktories

It is a survey of good characterised and lately came across bacterial protein pollutants. top investigators of the respective pollution overview a number of the molecular mechanisms of motion, starting from toxin-induced ADP-ribosylation as much as membrane perforation via pore-forming pollutants. Thy additionally describe the results on host body structure sooner than targeting capability functions as phone organic and pharmacological instruments for learn and clinical purposes. special descriptions of the technique comprise the engineering and use of changed and chimeric pollution for greater functionality. a fantastic advent to toxin constitution and features, in addition to a precious resource of method for researchers in molecular biology, pharmacology and experimental drugs.

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1994):Probing the structure-activity relationship of Escherichia coli LT-A by site-directed mutagenesis. In Mol. Microbiol. 14:51-60. Quiding M, Nordstrom I, Kilander A, et a/. (1991): Intestinal immune responses in humans. Oral cholera vaccination induces strong intestinal antibody responses and interferon-y production and evokes local immunological memory. In J. Clin. Invest. 88: 143- 148. Ramamurthy T, Garg S, Sharma R, et a/. (1993):Emergence of novel strain of Vibrio cholerae with epidemic potential in southern and eastern India.

3 Assay 1 :The G,, Assay This assay measures toxin-catalyzed transfer of ADP-ribose from NAD to a specific arginine(s) in G, the heterotrimeric GTP-binding protein subunit that is the stimulatory regulator of adenylyl cyclase. In the assay, the toxin catalyzes the transfer of [32P]ADP-ribosefrom NAD to G, or to other arginine-containing proteins. The reaction is stopped with ice-cold Z5 % TCA, and the precipitated proteins are recovered for SDS-PAGE and autoradiography. , 1989), labeled proteins are precipitated and collected on nitrocellulose filters for direct quantification of incorporated ADPribose.

Lebens M, Holmgren J (1994):Mucosal vaccines based on the use of cholera toxin B subunit as immunogen and antigen carrier. In Dev. Biol. Stand. 82:215-227. Lencer WI, de Almeida JB, Moe S, etal. (1993):Entry of cholera toxin into polarized human intestinal epithelial cells: Identification of an early brefeldin A-sensitive event required for A,-peptide generation. In J. Clin. Invest. 92:2941-2951. Lencer WI, Moe S, Rufo PA, et a/. (1995):Transcytosis of cholera toxin subunits across model human intestinal epithelia.

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