Biosurfactants: From Genes to Applications (Microbiology by Gloria Soberón-Chávez

By Gloria Soberón-Chávez

Biosurfactants, tensio-active compounds produced via dwelling cells, at the moment are gaining expanding curiosity because of their capability purposes in lots of varied commercial components during which to this point nearly solely artificial surfactants were used. Their distinctive constructions and features are only turning out to be favored. additionally, biosurfactants are thought of to be environmentally “friendly,” rather non-toxic and biodegradable. This Microbiology Monographs quantity bargains with the newest advances within the box of microbial biosurfactants, reminiscent of rhamnolipids, serrawettins, trehalolipids, mannosylerythritol lipids, sophorolipids, surfactin and different lipopeptides. each one bankruptcy studies the features of somebody biosurfactant together with the physicochemical houses, the chemical buildings, the position within the body structure of the manufacturing microbes, the biosynthetic pathways, the genetic legislation, and the capability biotechnological purposes.

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They are instead part of a dissociated fatty acid synthetase system, in which the individual reactions are catalyzed by separate proteins that are encoded by separate genes (Hoang and Schweizer 1997; White et al. 2005). The reference system for FAS II biochemistry is E. coli (White et al. 2005). In general, however, these enzymes are homologous among bacteria. For example, many isozymes of FabI, an enoyl-acyl carrier protein reductase, have been found in other bacteria such as FabL, FabV, and FabK (Zhu et al.

1998), another study proved that PHA-synthase negative mutants are still capable of RL production (Pham et al. 2004). Biosynthetic Link Between the Rhamnolipids Lipidic Moiety and Quorum Sensing Signal Molecules P. aeruginosa produces two classes of signal molecules, the acyl homoserine lactones (AHLs) and the 4-hydroxy-2-alkylquinolines (HAQs). The most abundant AHLs are N-(3-oxododecanoyl)-L-HSL and N-butanoyl-L-HSL, while the HAQs include 3,4-dihydroxy-2-heptylquinoline [Pseudomonas Quinolone Signal (PQS)] and its precursor 4-hydroxy-2-heptylquinoline (HHQ) (De´ziel et al.

Coli of dTDP-L-rhamnose restricts the production of mono-RLs in this species. By coexpression of the rhlAB and the rmlBDAC operons, the latter encoding the dTDP-L-rhamnose biosynthesis enzymes, they 42 A. Mohammad Abdel Mawgoud et al. generated an RL-producing E. 2 mg/L). In contrast, Wang et al. (2007) claimed RL production in E. coli expressing only rhlAB. Cha et al. (2008) reported the heterologous production of RLs in P. putida, using recombinant rhlABRI genes. 2 g/L of RLs was achieved. 2 Non-P.

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